Drug establishment – Drugo http://drugo.biz/ Fri, 27 May 2022 05:17:12 +0000 en-US hourly 1 https://wordpress.org/?v=5.9.3 https://drugo.biz/wp-content/uploads/2021/10/icon-24-120x120.png Drug establishment – Drugo http://drugo.biz/ 32 32 Establishment of an experimental model of a normal dog bladder organoid using a three-dimensional culture method https://drugo.biz/establishment-of-an-experimental-model-of-a-normal-dog-bladder-organoid-using-a-three-dimensional-culture-method/ Tue, 24 May 2022 15:02:24 +0000 https://drugo.biz/establishment-of-an-experimental-model-of-a-normal-dog-bladder-organoid-using-a-three-dimensional-culture-method/ This article was originally published here Biomed Pharmacologist. 2022 May 20;151:113105. doi: 10.1016/j.biopha.2022.113105. Online ahead of print. ABSTRACT Canine bladder cancer (BC) is predominantly muscle invasive (MI) with a poor prognosis, and its pathogenesis is close to that of humans. Three-dimensional (3D) organoid culture guarantees new knowledge about cancer diseases, including BC. Recently, we established […]]]>

This article was originally published here

Biomed Pharmacologist. 2022 May 20;151:113105. doi: 10.1016/j.biopha.2022.113105. Online ahead of print.

ABSTRACT

Canine bladder cancer (BC) is predominantly muscle invasive (MI) with a poor prognosis, and its pathogenesis is close to that of humans. Three-dimensional (3D) organoid culture guarantees new knowledge about cancer diseases, including BC. Recently, we established dog BC organoids (BCO) using their urine samples. BCO summarized the epithelial structures, characteristics, and drug sensitivity of dogs with BC. However, the organoids of normal canine bladder epithelium are not yet established. Therefore, the present study aimed to establish normal dog bladder organoids (NBO) to better understand the pathogenesis of dog BC and human MIBC. The established NBO underwent various analyses, including cellular marker expressions, histopathological structures, cancer-related gene expression patterns, and drug sensitivity. NBO could be produced noninvasively with continuous culture and recapitulated the structures and characteristics of normal canine bladder mucosal tissues. Different drug sensitivities were observed in each NBO. RNA sequencing analysis revealed that several new genes were modified in NBO compared to BCO. NBO showed higher expression of p53 and E-cadherin, but lower expression of MDM2 and Twist1 compared to BCO. These results suggest that NBO could be a promising 3D experimental model to study the developmental mechanisms of dog BC and human MIBC.

PMID:35605292 | DOI:10.1016/j.biopha.2022.113105

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Establishment and characterization of a novel cell line derived from dedifferentiated liposarcoma patients, NCC-DDLPS6-C1 https://drugo.biz/establishment-and-characterization-of-a-novel-cell-line-derived-from-dedifferentiated-liposarcoma-patients-ncc-ddlps6-c1/ Mon, 23 May 2022 06:00:00 +0000 https://drugo.biz/establishment-and-characterization-of-a-novel-cell-line-derived-from-dedifferentiated-liposarcoma-patients-ncc-ddlps6-c1/ This article was originally published here Hum cell. 2022 May 23. doi: 10.1007/s13577-022-00710-8. Online ahead of print. ABSTRACT Dedifferentiated liposarcoma (DDLPS) is morphologically characterized by well-differentiated liposarcomas associated with a high-grade non-lipogenic sarcoma and characterized molecularly by the coamplification of MDM2 and CDK4(12q14-15). DDLPS is very aggressive and effective systemic chemotherapy has not yet been […]]]>

This article was originally published here

Hum cell. 2022 May 23. doi: 10.1007/s13577-022-00710-8. Online ahead of print.

ABSTRACT

Dedifferentiated liposarcoma (DDLPS) is morphologically characterized by well-differentiated liposarcomas associated with a high-grade non-lipogenic sarcoma and characterized molecularly by the coamplification of MDM2 and CDK4(12q14-15). DDLPS is very aggressive and effective systemic chemotherapy has not yet been developed. In this study, we established a novel DDLPS cell line, NCC-DDLPS6-C1, as a potential tool for the development of novel therapies. NCC-DDLPS6-C1 cells were established from surgically resected tumor tissue from a patient with DDLPS. Amplification and overexpression of MDM2 and CDK4 were observed in NCC-DDLPS6-C1 cells. NCC-DDLPS6-C1 cells proliferated rapidly, invaded aggressively, and formed spheroids. Additionally, NCC-DDLPS6-C1 cells formed tumors in mice. These observations suggest that malignant potentials that may reflect the original features of DDLPS have been retained in NCC-DDLPS6-C1. Anticancer drugs that significantly reduced NCC-DDLPS6-C1 cell proliferation were identified by drug library screening. Thus, NCC-DDLPS6-C1 can recapitulate the original genotypes and phenotypes, and we conclude that the NCC-DDLPS6-C1 cell line is a useful resource for the study of DDLPS.

PMID:35604485 | DOI:10.1007/s13577-022-00710-8

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Kurt Schrader could be the next establishment Democrat toppled by a progressive challenger https://drugo.biz/kurt-schrader-could-be-the-next-establishment-democrat-toppled-by-a-progressive-challenger/ Wed, 18 May 2022 17:22:28 +0000 https://drugo.biz/kurt-schrader-could-be-the-next-establishment-democrat-toppled-by-a-progressive-challenger/ Schrader supported the decoupling of the bipartisan Infrastructure Act and social policy legislation called the Build Back Better Act, which was later stalled in the Senate due to Manchin’s opposition. During the most tense moments of these negotiations, he called Speaker Nancy Pelosi “a really terrible person” to a group of donors who supported the […]]]>

Schrader supported the decoupling of the bipartisan Infrastructure Act and social policy legislation called the Build Back Better Act, which was later stalled in the Senate due to Manchin’s opposition. During the most tense moments of these negotiations, he called Speaker Nancy Pelosi “a really terrible person” to a group of donors who supported the undermining of the Build Back Better Act, according to a book by New York Times journalists Alexander Burns and Jonathan Martin. (Pelosi later endorsed it.)

It was not the first time in recent years that Schrader has opposed the Democratic leadership. After the January 6, 2021, uprising at the United States Capitol, he told party leaders that he opposition to indictment former President Donald Trump, comparing it to a “lynching”. (A consulting firm cut ties with Schrader over the remark, and the congressman apologized for the comment and voted to impeach Trump.) Schrader also initially opposed Trump’s sweeping bill. Democrats coronavirus relief, the US bailout. In 2020, he voted against the PRO law, a labor law reform bill, provoking the ire of local labor organizations in his district. He then backed him up when he returned to the ground in 2021.

Schrader had the advantage in fundraising, raising over $2 million compared to around half a million raised by his opponent, and significantly outpaced McLeod-Skinner in the final weeks of the campaign. According to OpenSecrets, its biggest donors came from the pool of pharmaceutical companies and related corporate interests.

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Establishment candidate in the Colombian elections linked to the mafia https://drugo.biz/establishment-candidate-in-the-colombian-elections-linked-to-the-mafia/ Tue, 17 May 2022 09:26:29 +0000 https://drugo.biz/establishment-candidate-in-the-colombian-elections-linked-to-the-mafia/ A campaign manager for former President Alvaro Uribe and presidential candidate Federico Gutierrez has reportedly done business with narcos for years. Links between Gutierrez’s campaign and organized crime have been exposed by El Espectador newspaper and the election favorite. opposition senator Gustavo Petro. El Espectador revealed on Sunday that businessman Cesar Giraldo, aka “Calzones”, had […]]]>

A campaign manager for former President Alvaro Uribe and presidential candidate Federico Gutierrez has reportedly done business with narcos for years.

Links between Gutierrez’s campaign and organized crime have been exposed by El Espectador newspaper and the election favorite. opposition senator Gustavo Petro.

El Espectador revealed on Sunday that businessman Cesar Giraldo, aka “Calzones”, had ties to drug traffickers in the iconic coffee region for years.

Petro later accused Calzones of being behind a plan to assassinate the favorite during a field visit by organized crime group ‘La Cordillera’ earlier this month.


Citing assassination plot, Colombia’s electoral race favorite suspends rallies


The Cordillera is an organized crime group that has controlled most of the organized crime racketeering in the coffee region for the past 15 years.

Calzones allegedly did business with local narcos and became Uribe’s ‘special delegate’ in the town of Pereira during the same period, according to El Espectador.

President Ivan Duque (2nd L) and “Calzones” (2nd R)

The self-proclaimed entrepreneur is the founder of Uribe’s “Democratic Center” party in Pereira and one of the former president’s closest allies in the surrounding province of Risaralda.

Calzones is also a mob boss and the campaign coordinator for establishment candidate Federico Gutierrez, aka “Duque 2,” according to Petro.

He owns loans in the city of Pereira and is the head of the Duque 2 campaign, seeking the electoral majority in Pereira for Duque 2 to become president. I had to suspend the rally there because they said they were going to kill me, the same gunmen who collect the loans that the threatened woman has to pay at gunpoint at a daily rate of 20 % so aka Calzones can become a millionaire.

Senator Gustavo Petro

In a response, Uribe confirmed that Calzones “was a militant of our party” in Pereira, and did so “honourably”.

The former president urged the attorney general’s office to verify whether Petro’s alleged assassination plot was real.

You have to find out quickly if this is a whiff of Mr. Petro or if the conspiracy exists.

Former President Alvaro Uribe

Attorney General Francisco Barbosa, a subordinate of Uribe, announced an investigation almost immediately after the opposition leader went public with the alleged murder plot.

Petro Security handed over evidence of the alleged conspiracy to police nearly two weeks ago, after which the prosecution fell silent.


Petro Murder Plot Allegations Confirm Colombia’s Security Policy Failure


The newspaper El Espectador published the political, criminal and criminal connections that Calzone accumulated over the 20 years after starting his career with a food stand in the local market 20 years ago.

Since then, the self-proclaimed entrepreneur’s family has accumulated 127 properties, including luxury townhouses, a restaurant and a car dealership that sells some of Pereira’s most expensive vehicles, according to El Espectador.

Calzones acquired several properties from family members of drug traffickers associated with La Cordillera and its predecessor, the Norte del Valle Carte, according to El Espectador.

Pereira’s “entrepreneur” bought his first narcotics assets in 2005 from the wife and daughter of Jose Luis Vallejo, a fugitive drug trafficker at the time.

(Screenshot: El Espectador)

The Calzones continued buying properties from the Vallejo family until 2012, four years after the narco was extradited, El Espectador found.

Uribe’s partner in Pereira bought a farm from the family of Jonny Andres Valencia in 2015 after the drug trafficker was arrested in Brazil.

In 2017, Calzones reportedly purchased property from a former associate of La Cordillera boss “El Meme”, who was murdered in Pereira in 2015.

Former President Alvaro Uribe (L) and ‘Calzones’ (Image: Twitter)

While buying mafia properties, Calzones founded the Democratic Center party’s regional branch in Pereira and is said to have become Uribe’s “special delegate” in the coffee region.

In 2016, the mafia associate was the regional campaign coordinator for Uribe ahead of a referendum to reject a peace deal with the now defunct guerrilla group FARC.

Calzones met Duque in 2017 after the mob associate became the regional coordinator for the president’s 2018 campaign.

Calzones was invited to Duque’s inauguration along with “El Ñeñe”, the late money launderer from Marcos Figueroa’s drug trafficking organization who had campaigned for the president in the north of the country.

Gutierrez campaign rally in Pereira on Sunday. (Picture: Twitter)

The mafia associate’s 24-year-old son, Cristian Camilo Giraldo, is currently one of Gutierrez’s regional campaign bosses in Pereira.

The rally of establishment candidates in Pereira on Sunday was marred by evidence that regional businesses were offering residents money to attend the rally.

Gustavo Villegas

The scandal caused by his campaign in Pereira is not the first for Gutierrez, who was mayor of Medellin between 2015 and 2019.

In 2018, former Gutierrez security secretary Gustavo Villegas was sent to prison after prosecutors found evidence that the Medellin dynasty politician had ties to “La Oficina de Envigado”.


Medellin security secretary arrested for organized crime


This organized crime group was originally founded by late drug lord Pablo Escobar, whose Medellin cartel was close to the Uribe family.


How the Former Colombian President Helped Launch the Medellin Cartel


The “Fico” administration has also awarded government contracts to a firm that belonged to the family of late Medellin cartel trafficker Ramiro Arturo Correa, the investigative journalism website Cuestion Publica revealed earlier this year.

The Correa clan had previously struck deals with Gutierrez’s predecessor and election rival, Sergio Fajardo, and former Medellin mayor Luis Perez, who dropped out of the race last week.

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Establishment Labs (NASDAQ:ESTA) and Milestone Scientific (NYSE:MLSS) Head to Head Survey https://drugo.biz/establishment-labs-nasdaqesta-and-milestone-scientific-nysemlss-head-to-head-survey/ Sun, 15 May 2022 20:27:50 +0000 https://drugo.biz/establishment-labs-nasdaqesta-and-milestone-scientific-nysemlss-head-to-head-survey/ Establishment Labs (NASDAQ:ESTA – Get Rating) and Milestone Scientific (NYSE:MLSS – Get Rating) are both small-cap medical companies, but which is the better stock? We’ll compare the two companies based on valuation strength, risk, earnings, institutional ownership, profitability, dividends, and analyst recommendations. Profitability This table compares the net margins, return on equity, and return on […]]]>

Establishment Labs (NASDAQ:ESTA – Get Rating) and Milestone Scientific (NYSE:MLSS – Get Rating) are both small-cap medical companies, but which is the better stock? We’ll compare the two companies based on valuation strength, risk, earnings, institutional ownership, profitability, dividends, and analyst recommendations.

Profitability

This table compares the net margins, return on equity, and return on assets of Establishment Labs and Milestone Scientific.

Net margins Return on equity return on assets
Establishment laboratories -29.77% -70.10% -27.60%
Scientific milestone -74.26% -39.61% -32.63%

Institutional and Insider Ownership

77.8% of Establishment Labs shares are held by institutional investors. By comparison, 7.8% of Milestone Scientific shares are held by institutional investors. 13.1% of Establishment Labs stock is held by company insiders. By comparison, 21.4% of Milestone Scientific stock is held by company insiders. Strong institutional ownership indicates that hedge funds, large fund managers, and endowments believe a stock will outperform the market over the long term.

Benefits and evaluation

This chart compares the gross revenue, earnings per share, and valuation of Establishment Labs and Milestone Scientific.

Gross revenue Price/sales ratio Net revenue Earnings per share Price/earnings ratio
Establishment laboratories $126.68 million 10.61 -$41.14 million ($1.66) -33.46
Scientific milestone $10.31 million 5.55 -$6.82 million ($0.10) -8.40

Milestone Scientific has lower revenues, but higher revenues than Establishment Labs. Establishment Labs trades at a lower price-to-earnings ratio than Milestone Scientific, indicating that it is currently the more affordable of the two stocks.

Analyst Recommendations

This is a breakdown of recent ratings and target prices for Establishment Labs and Milestone Scientific, as provided by MarketBeat.com.

Sales Ratings Hold odds Buy reviews Strong buy odds Rating
Establishment laboratories 0 1 3 0 2.75
Scientific milestone 0 0 0 0 N / A

Establishment Labs currently has a consensus target price of $101.17, indicating a potential upside of 82.15%. Given the higher potential upside of Establishment Labs, equity research analysts clearly believe that Establishment Labs is more favorable than Milestone Scientific.

Risk and Volatility

Establishment Labs has a beta of 1.08, suggesting its stock price is 8% more volatile than the S&P 500. By comparison, Milestone Scientific has a beta of 1.45, suggesting its stock price is 45% more volatile than the S&P 500.

Summary

Establishment Labs beats Milestone Scientific on 7 out of 12 factors compared between the two stocks.

About Institutional Laboratories (Get a rating)

Establishment Labs Holdings Inc., a medical technology company, manufactures and markets medical devices for aesthetic and reconstructive plastic surgery. The company primarily offers silicone gel-filled breast implants under the Motiva Implants brand. It also provides Motiva Ergonomix and Motiva Ergonomix2 soft silicone gel-filled round breast implants; and Motiva Flora Tissue Expander, a breast tissue expander, and also distributes the Puregraft line of products for the harvesting and redistribution of autologous adipose tissue. The company sells its products through exclusive distributors and a direct sales force in Europe, Latin America, Asia-Pacific and internationally. Establishment Labs Holdings Inc. was incorporated in 2004 and is headquartered in Alajuela, Costa Rica.

About Milestone Scientific (Get a rating)

Milestone Scientific Inc. designs, develops and markets diagnostic and therapeutic injection technologies, as well as devices for medical, dental and cosmetic use in the United States, China and around the world. The Company operates in two segments, dental and medical. Its products include CompuDent and STA Single Tooth Anesthesia System which are used for all dental procedures requiring local anesthesia. The company also offers CompuFlo, a computer-controlled drug delivery system for the painless administration of drugs, anesthetics and other medications, as well as the aspiration of bodily fluids or previously injected substances; and disposable injection handpiece for tactile control during injection. Additionally, it provides CompuFlo Epidural, a computer-controlled anesthesia system for use in various medical applications. Additionally, the Company offers CompuMed for use in various medical procedures performed in the fields of plastics, hair restoration and colorectal surgery, as well as podiatry, dermatology, orthopedics and various others. disciplines. Milestone Scientific Inc. was incorporated in 1989 and is headquartered in Livingston, New Jersey.



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Kick-off event for the creation of a counter-terrorism law enforcement forum https://drugo.biz/kick-off-event-for-the-creation-of-a-counter-terrorism-law-enforcement-forum/ Fri, 13 May 2022 19:38:00 +0000 https://drugo.biz/kick-off-event-for-the-creation-of-a-counter-terrorism-law-enforcement-forum/ On May 11-12 in Berlin, the Departments of State and Justice launched the Counterterrorism Law Enforcement Forum (CTLEF), co-hosted by the German government. CTLEF, which focuses on countering racially or ethnically motivated violent extremism (REMVE) globally, has brought together law enforcement, prosecutors and other criminal justice practitioners from Europe, North and South America and the […]]]>

On May 11-12 in Berlin, the Departments of State and Justice launched the Counterterrorism Law Enforcement Forum (CTLEF), co-hosted by the German government. CTLEF, which focuses on countering racially or ethnically motivated violent extremism (REMVE) globally, has brought together law enforcement, prosecutors and other criminal justice practitioners from Europe, North and South America and the Indo-Pacific, alongside specialists from INTERPOL, Europol, the International Institute for Justice and the Rule of Law, the United Nations Office Against Drugs and Crime (UNODC) and other multilateral organizations.

The inaugural CTLEF meeting increased awareness and information sharing among law enforcement and criminal justice partners on global REMVE activity, including transnational linkages between and among REMVE actors. Participants also learned about recent law enforcement actions taken by governments to disrupt the global activity of REMVE actors.

Officials from the United States Departments of State, Justice and Treasury, as well as the Federal Bureau of Investigation, participated. The CTLEF will meet again in spring 2023.

/Public release. This material from the original organization/authors may be ad hoc in nature, edited for clarity, style and length. The views and opinions expressed are those of the author or authors. See in full here.

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India and establishing a direct correlation between GDP and GNH https://drugo.biz/india-and-establishing-a-direct-correlation-between-gdp-and-gnh/ Thu, 12 May 2022 09:38:06 +0000 https://drugo.biz/india-and-establishing-a-direct-correlation-between-gdp-and-gnh/ We are aware that the Indian economy is basically divided into 3 parts: Agriculture; Services; Industry. Agriculture and industry together account for about 33-35% (one third) of GDP and services account for the remaining 65-67% (two thirds). For some reason the industry hasn’t taken off in India and in my opinion looking at India’s ‘free-thinking’ […]]]>

We are aware that the Indian economy is basically divided into 3 parts:

  1. Agriculture;
  2. Services;
  3. Industry.

Agriculture and industry together account for about 33-35% (one third) of GDP and services account for the remaining 65-67% (two thirds). For some reason the industry hasn’t taken off in India and in my opinion looking at India’s ‘free-thinking’ culture, the industry which requires some regulated nature of work is not suited to our way of thinking and our behavior.

However, over the past decade of years, the Indian economy could be observed with additional growth areas which can be additional growth engines to the above 3:

  1. Financial inclusion through and with IT services and applications;
  2. IT services improving the agricultural base through the sharing of agricultural information through applications and the construction of large agricultural processing plants near major mandis and agricultural markets;
  3. Health sector – pharmaceutical manufacturing plants, hospitals, IT services facilitating access to health in remote areas through internet access;
  4. Education infrastructure – educational institutions prepare Indians for the reality of working in India with its challenges. For too long we have created professionals for other countries;
  5. Logistics hubs and storage facilities facilitating better distribution of products in terms of speed and cost of delivery;
  6. Transport sector – Rail, maritime, air and road transport facilities.

All of the 6 additional growths above are about to kick in together, giving our GDP a serious boost. How we manage this economic recovery is the real challenge. Every 100 basis point increase in the percentage of GDP leads to a huge increase in utility needs such as electricity, oil and gas, water, and additional infrastructure needs. Managing these expected increases is a huge challenge in itself. Somehow we seem stuck at the glass ceiling growth rate of 7.5-8.5% per year. We are struggling to move to a GDP growth rate of 9.0 to 9.5%. It is because we have reached a plateau on agriculture and industry and we do not see how to do things differently. In fact, if agriculture alone is properly managed (despite the vested interests of big landowners and powerful traders who don’t want change), India’s economy can get a boost of at least 50 basis points. (half %).

The significance of these 7 new subsets of growth is that not only do they add to GDP (Gross Domestic Product), but they have the potential to improve GNH (Gross National Happiness). For too long in India we have had empty arguments that GDP growth does not create jobs, that GDP growth outcomes have not percolated deep enough, that growth is uneven and creates more inequalities, etc.

These 6 additional growth drivers are capable of increasing Gross National Happiness thanks to:

  1. Improved farm incomes and financial inclusion will bring more Indians into the formalized economy and everyone will become part of the national growth process. Our experiences with Jan Dhan accounts, Unified Payment Interface (UPI), direct transfer of benefits (subsidies and product prices credited to bank accounts) have created an atmosphere in which the vying citizen feels that he receives his due;
  2. It is well known that educated and healthy citizens are much more productive at work. Clearly, the jobs these citizens will do are higher quality jobs, which translates to mental satisfaction and better pay. Our physical infrastructure for health and education is poor. However, meeting the investment needs of these two sectors can generate benefits that are multiples of the investment costs;
  3. We must understand that the citizen does not need to come to work from his place of residence. The Job can now come back to him. After the covid 19 pandemic, this is what seems to be happening. The need for centralized workplaces seems to have diminished. Employers are striving to harness talent in hitherto smaller towns – providing the bliss of staying home and not commuting for the employee and saving office maintenance costs for the employee. ’employer. For the first time, we see that the rush to megacities can be controlled while small towns and villages grow, leading to citizen happiness;
  4. Transporting and storing goods and materials is a major challenge. Fortunately, the improvement of the physical facilities of airports, stations and trains, of the roads and of the vehicles that circulate there, and the development of transport by water have contributed to creating new jobs, additional services and bringing people closer together. customer markets.

All of the above activities not only add to GDP, but increase GNH which itself increases GDP. A satisfied people is a more productive people.

Unfortunately, I don’t see the industry segment contributing significantly to GDP and GNI growth. High-end technology-driven industry will grow, the middle segment of industry will continue to be squeezed, the lower segment of MSME-type industry will survive but never prosper, India has missed out on the revolution of industry. For it to catch up with the industry, a huge shift in the mindsets of employers and employees is needed. The employer should stay away from seeking protection and subsidies, the employee should not make unreasonable compensation demands and fanciful facilities and worry about productivity.

We must not miss the computer/financial/agricultural revolution which is waiting for the right direction to move it forward.

India is poised to see a better direct correlation between growth in terms of numbers (GDP) and growth in terms of welfare and well-being (GNH). The impetus to succeed must come from us.



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The opinions expressed above are those of the author.



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Modified protocol for establishing a model of intracranial arterial dolichoectasia by injection of elastase into the cerebellomedullary cistern in mice https://drugo.biz/modified-protocol-for-establishing-a-model-of-intracranial-arterial-dolichoectasia-by-injection-of-elastase-into-the-cerebellomedullary-cistern-in-mice/ Fri, 06 May 2022 06:00:00 +0000 https://drugo.biz/modified-protocol-for-establishing-a-model-of-intracranial-arterial-dolichoectasia-by-injection-of-elastase-into-the-cerebellomedullary-cistern-in-mice/ This article was originally published here Neurol before. 2022 Apr 18;13:860541. doi: 10.3389/fneur.2022.860541. eCollection 2022. ABSTRACT BACKGROUND AND PURPOSE: This study aimed to construct an animal model of intracranial arterial dolichoectasis (IADE) by applying the modified modeling protocol. MATERIALS AND METHODS: Twenty-five milliunits of elastase and inactivated elastase were respectively injected into the cerebellar-medullary cistern […]]]>

This article was originally published here

Neurol before. 2022 Apr 18;13:860541. doi: 10.3389/fneur.2022.860541. eCollection 2022.

ABSTRACT

BACKGROUND AND PURPOSE: This study aimed to construct an animal model of intracranial arterial dolichoectasis (IADE) by applying the modified modeling protocol.

MATERIALS AND METHODS: Twenty-five milliunits of elastase and inactivated elastase were respectively injected into the cerebellar-medullary cistern of 60 C57/BL6 mice which were divided into experimental groups (EG, not = 30) and control group (CG, not = 30) using a computerized random order generator. The modified modeling protocol clarified these aspects, including three-dimensional brain parameters of mouse head fixation, head tilt angle, conical ear fixed position, holding technique needle depth, needle entry depth, liquid drug backflow prevention and elastase storage conditions. And this was observed for the following parts such as mortality, inflammatory factors, craniocerebral artery scintigraphy, vascular tortuosity index, artery diameter, cerebrovascular pathology.

RESULTS: In the stages studied differently, the total mortality of mice in EG was 20%. ELISA showed that levels of matrix metalloproteinase-9 (MMP-9) and tumor necrosis factor-α (TNF-α) in peripheral blood increased significantly after modeling. Angiography indicated that 100% of IADEs in the EG were observed and that the diameter and tortuosity index of the basilar artery were significantly increased (P < 0.01). Histological processing and EVG staining showed the disrupted inner elastic lamina, atrophied muscle layer, and hyalinized connective tissue of the basilar artery with the tunica of the vessel wall in EG. Micro-computed CT scans reported that the craniocerebral arteries of EG mice were remarkably elongated, tortuous and dilated.

CONCLUSION: The modified modeling protocol can reduce mortality, improve the success rate, and provide a stable animal model for IADE.

PMID:35518204 | PMC: PMC9062172 | DOI:10.3389/fneur.2022.860541

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Robert Menendez Jr. runs with the support of the establishment in the 8th arrondissement https://drugo.biz/robert-menendez-jr-runs-with-the-support-of-the-establishment-in-the-8th-arrondissement/ Thu, 05 May 2022 20:13:49 +0000 https://drugo.biz/robert-menendez-jr-runs-with-the-support-of-the-establishment-in-the-8th-arrondissement/ Robert Menendez Jr. is pushing for policies that will help workers and families in the 8th Ward. Photo by Mark Koosau. For politicians and other observers watching the race for the 8th congressional district closely this year, Robert Menendez Jr. needs no introduction. For one, he is the son of U.S. Senator Robert Menendez, a […]]]>

Robert Menendez Jr. is pushing for policies that will help workers and families in the 8th Ward. Photo by Mark Koosau.

For politicians and other observers watching the race for the 8th congressional district closely this year, Robert Menendez Jr. needs no introduction.

For one, he is the son of U.S. Senator Robert Menendez, a powerful and influential figure in Hudson County and New Jersey politics. Second, he has the backing of the county’s Democratic establishment, as well as state figures such as Gov. Phil Murphy and Sen. Cory Booker, to potentially take over from incumbent Representative Albio Sires.

As for why he’s running for the Hudson County seat in Congress, Menendez Jr. said in an interview with the Hudson Reporter that the policies it advocates will help workers and families.

“I think when you look at the challenges that people are facing, especially the 8th congressional district, which is largely a working-class and middle-class community, the issues have an impact on child care, l early childhood education, affordability, uplifting workers by supporting organized work, creating more opportunities for [them]” he said.

“I think people want to see an investment in our communities, our families and our well-being.”

Affordability, healthcare and education

Menendez Jr., an attorney, has worked at Lowenstein Sandler for more than a decade and most recently served as its attorney since 2018. He has also served as commissioner at the Port Authority of New York and New Jersey since June of last year. , and is also a trustee on the board of directors of the Hudson School in Hoboken.

Menendez Jr., who currently lives in Jersey City, said there’s a sense of obligation “that we can’t wait for someone else to move on” after the Jan. 6 insurgency l ‘last year.

“I thought at that time, a year ago, that we were going to turn the page on a dark chapter in the history of our country during years of Trump presidency,” he said. “Instead, what we saw was an insurrection and an attack on the Capitol.”

The biggest challenge he sees for voters in the 8th arrondissement is affordability, noting rising inflation in the country. “Even with the salary increases, people still feel like they’re not progressing,” he said. “This affordability extends to housing, access to health care, [and] education.”

While campaigning for the Democratic nomination, Menendez Jr. has offered a liberal policy platform to address these issues, such as lowering the cost of prescription drugs, expanding access to Medicare, and Medicaid and Universal Pre-K.

Menendez Jr. said there was a sense of obligation after the Jan. 6 uprising last year. Photo provided by Menendez for Congress.

Going deeper into education, he advocates investing federal funds in creating school facilities, and also looking at job training and workforce development to create different pathways for those outside of four-year college. He also supports up to $50,000 in student debt relief.

On housing, Menendez Jr. said he would work on Democratic packages that would lead to “greater investment” in affordable housing and livable communities. “Not just thinking about affordability, but thinking about housing our seniors, people with disabilities and making sure that any public investment in housing, there is also a connection to public transport,” he said. .

For immigration, Menendez Jr. has supported “comprehensive” immigration reform, including fully integrating into American society, providing a pathway to citizenship and reworking the country’s visa programs so they can attract more people and provide more opportunities for growth in the country.

“We have an aging population and a shrinking workforce, so we’re going to have a problem in our labor market,” he said. “In my view, immigration is a moral and humanitarian issue, but we should also view it as an economic issue, and if we are to drive real economic growth in this country, we need to provide a pathway to citizenship so that people have a full say in what we offer as a country.

Asked about his stance on federal Immigration and Customs Enforcement (ICE), Menendez Jr. said local governments shouldn’t work with them when dividing families, but said they play a role in monitoring the country’s customs and borders.

Your kingdom come, your will be done

A sticking point for many observers throughout the primary is how quickly the Democratic establishment coalesced around Menendez Jr. as soon as Sires announced his retirement in December, even before he had officially announced his candidacy.

Some people, like progressive candidate David Ocampo Grajales, have criticized establishment movements, mainly because of what they say is senior Menendez’s influence.

Asked about the critics, Menendez Jr. did not respond directly and said they were tuning in and bringing their message to voters. “In my experience, people are very positive when you go out and talk to constituents.”

As for how he plans to deal with the gridlock and polarization Congress has become known for, he acknowledges it will be a challenge, but said they should take a bipartisan approach to engaging with people who disagree with them.

Menendez Jr. said he would take a bipartisan approach to dealing with gridlock and polarization in Congress. Photo provided by Menendez for Congress.

If Democrats lose their majority in the House, Menendez Jr. says it will be important to continue to seek solutions at the legislative level for voters and to provide access to federal resources.

For the committees he would like to serve on, he sees “realistically” looking at the transportation and infrastructure committee for its first term, noting the challenges in the district and the amount of infrastructure funds coming into the state, and has said having a seat at the table and directing the money to the district would be “extremely important.”

With nearly a month to go before the June primary, Menendez’s message earlier, he says, is that “we hear you, we see you, we know this has been a difficult time for you as it has been. been difficult for your family and communities.

“We want to go to work hard, every day, [and] provide the best possible solutions for you, for your families, [and] for our communities.

For updates on this story and others, check out hudsonreporter.com and follow us on Twitter @hudson_reporter. Mark Koosau can be reached at mkoosau@hudsonreporter.com or on his Twitter @snivyTsutarja.

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Madison Cawthorn says ‘establishment’ is trying to kill her political career / Queerty https://drugo.biz/madison-cawthorn-says-establishment-is-trying-to-kill-her-political-career-queerty/ Thu, 28 Apr 2022 14:30:09 +0000 https://drugo.biz/madison-cawthorn-says-establishment-is-trying-to-kill-her-political-career-queerty/ Madison Cawthorn (Photo: Gage Skidmore, via CC BY-SA 2.0) Scandal-hit lawmaker Madison Cawthorn posted a two-and-a-half-minute video on Instagram saying that “the establishment and RINOS [Republican In Name Only]are there to destroy his chances of being re-elected. According to the North Carolina GOP politician, there will be a “drip campaign” of bad stories about him […]]]>
Madison Cawthorn (Photo: Gage Skidmore, via CC BY-SA 2.0)

Scandal-hit lawmaker Madison Cawthorn posted a two-and-a-half-minute video on Instagram saying that “the establishment and RINOS [Republican In Name Only]are there to destroy his chances of being re-elected.

According to the North Carolina GOP politician, there will be a “drip campaign” of bad stories about him over the next few weeks.

Why? Because there are forces at work for him not to be re-elected, because according to Cawthorn, “statistically, it’s virtually impossible to beat an incumbent of Congress after winning his first re-election…that’s why you’re starting to see this coordinated attack among many freshman members of Congress who are hardline conservatives running for re-election.

In an accompanying caption, Cawthorn says, “Senators Rino and establishment pawns want us to go back to the days before Trump. They want to go back to the days when they didn’t take offense, didn’t fight for the next generation, didn’t try to dismantle the excesses of the federal government, we’ll never go back.

“That’s why the establishment is trying to crush me and the others, they’re attacking what they fear. Forward!”

Cawthorn says even other Republicans are starting to say “ridiculous and salacious” things about him in North Carolina. “They say we don’t serve our district, which I think is nonsense.”

He continued, “I make no apologies for ensuring that we cultivate a following across all platforms, trying to reach my generation, which is the most drugged, addicted, depressed, suicidal generation that has ever never lived. And it’s because of these radical liberal policies and the indoctrination that’s going on in our schools.

Cawthorn may be referring to an attack on him earlier in the week by Republican Senator Chuck Edwards, who wants to take Cawthorn’s seat. Edwards released a campaign video saying Cawthorn is doing zilch for his district and is more concerned about his Instagram posts.

Cawthorn concluded, “When I say drip campaign…they’re going to run an attack article every one or two days, just trying to kill us with death by a thousand cuts. And that’s really their main strategy.

Controversy has dogged Cawthorn, 26, since he first announced his candidacy. The youngest elected to Congress in November 2020, he made headlines with his controversial opinions and speeches, such as telling moms to raise hyper-masculine “monsters.”

While campaigning for election, Cawthorn said the car accident that left him in a wheelchair derailed his place to attend naval college. It actually turned out that he had been rejected by the naval school before the accident took place.

Last December, he gave a speech at the Turning Point USA conference urging young conservatives to drop out of school and marry young. A week later, he announced that he and his wife Cristina were separating after just eight months of marriage.

Related: Oh oh! Madison Cawthorn’s Bad Week Just Got Worse

For the past few weeks, he’s been making headlines for all the wrong reasons. Last month, he was charged with driving with a revoked license. A court appearance on the matter is expected in the coming weeks.

Then he said on a podcast that high-profile Republican figures invited him to orgies in Washington DC and that he saw anti-drug crusaders taking cocaine.

On Friday, Politico posted photos of Cawthorn wearing women’s lingerie at a party. Cawthorn says it was just a fun get-together aboard a cruise ship, long before he was a congressman.

This week, Cawthorn was cited for possession of a loaded handgun at Charlotte Douglas International Airport. This is the second time he has “accidentally” tried to carry a gun on a plane.

Related: Madison Cawthorn Just Got Arrested At The Airport…again

It sure seems like Cawthorn doesn’t need outside help when it comes to wrecking his own career.

North Carolina’s Republican primary takes place on May 17.

People had a lot to say about the video on Twitter.

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